Competent Cells
INTACT GENOMICS INC ig 10B Chemically Competent Cells (High Efficiency) 48x50µl
Intact Genomics ig 10B chemically competent cells (E. coli) are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes & Storage: ig 10B competent cells: -80 ºC pUC19 control DNA: -20 ºC Recovery medium: 4 ºC
INTACT GENOMICS INC DirectPlate DH5-alpha Chemically Competent Cells 36x50µl
Intact Genomics (ig) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. It's a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ DH5-Alpha chemically competent E. coli cells provide higher transformation efficiency than any competitor’s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
INTACT GENOMICS INC DirectPlate TG1 Phage Display Chemically Competent Cells
Intact Genomics (ig) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ TG1 Phage Display chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ TG1 Phage Display chemically competent E. coli cells provide higher transformation efficiency than any competitor’s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning, phage display libraries and sub-cloning.Product Includes and Storage:DirectPlate™ TG1 Phage Display competent cells: -80 ºCpUC19 control DNA: -20 ºC
INTACT GENOMICS INC AGL1 Agrobacterium Chemically Competent Cells 6x50µl
Intact Genomics Agrobacterium tumefaciens AGL1 (AGL-1) cells are optimized for the highest transformation efficiencies which are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicillin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for the transfer of genetic material into a host plant’s genome. Therefore, this system is often used for the Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.
INTACT GENOMICS INC JM109 Chemically Competent Cells 12x200µl
Intact Genomics JM109 Chemically Competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.ig™ JM109 chemically competent cells have the following features: • recA– and endA– to minimize recombination and improve the quality of plasmid DNA. • Contains F’ episome for blue/white screening.Product Components & Storage:1) ig™ JM109 competent cells: -80 ºC2) pUC19 control DNA: -20 ºC3) Recovery medium: 4 ºC
INTACT GENOMICS INC BL21(DE3) Chemically Competent Cells 12x50µl
Intact Genomics BL21(DE3) Chemically Competent E. coli Cells are suitable for transformation and routine protein expression.BL21(DE3) Chemically Competent Cells have the following features: Widely used host background T7 Expression Strain Deficient in both lon (1) and ompT proteases Resistant to phage T1 (fhuA2) B StrainProduct Includes and Storage:BL21(DE3) Competent Cells: -80 ºCpUC19 Control DNA: -20 ºCRecovery Medium: 4 ºC
INTACT GENOMICS INC HB101 Electrocompetent Cells 24x50μl
Intact Genomics (ig) HB101 electrocompetent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening.Product Include and Storage: ig HB101 electrocompetent cells: -80 ºCpUC19 control DNA: -20 ºCRecovery medium: 4 ºC
INTACT GENOMICS INC Ar.A4 Agrobacterium Chemically Competent Cells 18x50µl
Intact Genomics (ig) Ar.A4 Agrobacterium Chemically Competent cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes (kan R) Ar A4 Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons, and some gymnosperms. Ar.A4 Agrobacterium Chemically Competent Cells are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, carrot, and other plants. Ar.A4 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays kanamycin resistance.Product Includes and Storage:Ar.A4 Agrobacterium Chemically Competent Cells: -80 ºCpIG7spe control DNA: -20 ºCAgro Recovery medium: 4 ºC
INTACT GENOMICS INC TG1 Phage Display Electrocompetent Cells 12x100µl
Intact Genomics TG1 Phage Display ElectroCompetent Cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.Intact Genomics TG1 Phage Display ElectroCompetent Cells have the following features: • ≥4 x 10^10cfu/µg efficiency with electroporation. • Amber suppressor strain (supE)Product Include and Storage: TG1 phage display electrocompetent cells: -80 ºCpUC19 control DNA: -20 ºCRecovery medium: 4ºC
INTACT GENOMICS INC TG1 Phage Display Electrocompetent Cells 12x50µl
Intact Genomics TG1 Phage Display ElectroCompetent Cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.Intact Genomics TG1 Phage Display ElectroCompetent Cells have the following features: • ≥4 x 10^10cfu/µg efficiency with electroporation. • Amber suppressor strain (supE)Product Include and Storage: TG1 phage display electrocompetent cells: -80 ºCpUC19 control DNA: -20 ºCRecovery medium: 4ºC
INTACT GENOMICS INC DirectPlate DH5-alpha Chemically Competent Cells 1x96 well plate - 50µl/well
Intact Genomics (ig) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. It's a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ DH5-Alpha chemically competent E. coli cells provide higher transformation efficiency than any competitor’s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
INTACT GENOMICS INC MG1655 Chemically Competent Cells 12x200µl
Intact Genomics (ig) MG1655 Chemically Competent Cells are suitable for routine DNA transformation and other research purposes. MG1655 is the “wild type” K-12 strain with minimal genetic manipulation having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange (1).ig MG1655 chemically competent cells have the following genomic features: • Cured of the temperate bacteriophage lambda and F plasmid • A frameshift at the end of rph • ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II • rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis
INTACT GENOMICS INC ig ccdB Resist™ Electrocompetent E. coli Cells 6x50μl
Intact Genomics (ig) ccdB Resist™ ElectroCompetent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ccdB Resist™ cells are capable of propagation of plasmids containing the ccdB gene. Intact Genomics ccdB Resist™ ElectroCompetent Cells provide superb transformation efficiency, higher than any competitor’s similar product, allowing for increased opportunity for experimental success.ig ccdB Resist™ Electrocompetent Cells have the following features: ccdB Resist™ allows for cloning of methylated genomic sequences Stabilizes retroviral and direct repeat sequences including HIV High transformation efficiency allows aids in cloning rare sequences May be used for plasmids > 20 kb endA1 mutation increases plasmid yield significantlyProduct Include and Storage:ig ccdB Resist™ ElectroCompetent Cells: -80 ºCpUC19 control DNA: -20 ºCRecovery medium: 4 ºC
INTACT GENOMICS INC C58C1 Agrobacterium ElectroCompetent Cells 6x50µl
Intact Genomics (ig) C58C1 Agrobacterium ElectroCompetent Cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. These competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin-resistant.Useful for transgenic operations that involve potatoes, astragalus, rosaceae, tobacco and other plants.Product Includes and Storage:C58C1 Agrobacterium ElectroCompetent Cells: -80 ºCpCAMBIA1391z control DNA: -20 ºCAgro Recovery medium: 4 ºC
INTACT GENOMICS INC ig XL1 Blue Max Chemically Competent Cells 24x50µl
Intact Genomics (ig) XL1 Blue Max chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. These cells have the capability to allow for the preparation of high quality plasmid DNA, single strand rescue of phagemid DNA and blue/white screening. XL1 Blue Max cells provide superb transformation efficiency, significantly higher than any competitors similar product, allowing for increased opportunity for experimental success.ig XL1 Blue Max chemically competent cells have the following features: XL-1 Blue Max cells are tetracycline resistant. XL1-Blue Max cells are endonuclease (endA) deficient XL1-Blue Max cells recombination (recA) deficient, improving insert stability Cleavage of cloned DNA by the EcoK endonuclease system is prevented by the hsdR mutation. Blue-white color screening via the lacIq Z∆M15 gene on the F´ episome